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Procedures and guides

Printable procedures with figures computed by FIB-view. Free to use for training; follow your facility's procedures and the manufacturer's documentation.

SOP-01 · TEM lamella lift-outEucentric height, electron- and ion-beam Pt cap, bulk trenches, cleaning cross-sections, J-cut, lift-out, thinning with over-tilt, 5 and 2 kV polishing, troubleshooting and a checklist. With gallium or plasma ions. 11 pages, PDF.

SOP-02 · Python scriptingThe exhibit's Python IDE, the fibview package (headless or live), the calls, milling and deposition from Python, testing automation with challenges, batch data and regression tests. 8 pages, PDF.

SOP-03 · Cryo lamella for cryo-ETFrozen cells on a grid: cryo stage and anticontaminator, sputter coat, cold GIS Pt, milling angle, micro-expansion joints, rough and fine milling, polishing to 200 nm without devitrifying, final coat; gallium vs argon vs xenon damage, troubleshooting, cryo slice & view and a checklist. 12 pages, PDF.

HTML versions: SOP-01 · SOP-02 · SOP-03. For the microscope's alignment and AI agents, see the TEM-view guides.